The spontaneous hemin release from Lumbricus terrestris hemoglobin
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چکیده
منابع مشابه
Ceramide glycanase from the earthworm, Lumbricus terrestris.
Ceramide glycanase (CGase) is an enzyme that cleaves the linkage between the sugar chain and the ceramide. To make this enzyme readily available, we have developed a simple method for preparing it from the earthworm, Lumbricus terrestris. The method involves Bio-Gel A-0.5m, octyl-Sepharose and p-aminophenylthiogalactoside-agarose column chromatography. By gel filtration, the molecular mass of e...
متن کاملStoichiometry of subunits and heme content of hemoglobin from the earthworm Lumbricus terrestris.
The extracellular hemoglobin (Hb) of the earthworm, Lumbricus terrestris, has four major O2-binding chains, a, b, c (forming a disulfide-linked trimer), and d ("monomer"). Additional structural chains, "linkers," are required for the assembly of the approximately 200-polypeptide molecule. The proportion of linker chains had been reported to be one-third of the total mass on the basis of densito...
متن کاملThe structure of the gene encoding chain c of the hemoglobin of the earthworm, Lumbricus terrestris.
The complete nucleotide sequence of the gene for chain c of hemoglobin of the earthworm Lumbricus terrestris has been determined. The sequence of 4037 base pairs (bp) includes about 310 bp of 5'-flanking sequence and 110 bp 3' to the poly(A) site. Comparison of cDNA and genomic sequences shows four silent differences in codons that suggest the presence of at least two genes. The coding sequence...
متن کاملThe extracellular hemoglobin of the earthworm, Lumbricus terrestris. Determination of subunit stoichiometry.
The giant extracellular hemoglobin of the earthworm, Lumbricus terrestris, has four major O2-binding chains, a, b, and c (forming a disulfide-linked trimer) and d ("monomer"). Participation of additional "linker" chains L1, L2, and L3 is necessary for the assembly of the approximately 3,900+ kDa two-tiered hexagonal structure. We have determined the proportions of linker chains, trimer, and cha...
متن کاملNon-heme protein in the giant extracellular hemoglobin of the earthworm Lumbricus terrestris.
The protein/heme mass ratio for the extracellular hemoglobin of the earthworm Lumbricus terrestris has been redetermined. We find a value of 19,000 g of protein per mol of heme. Four major, heme-containing chains (a, b, c, and d), present in equal proportions, have a total molecular mass, with four hemes, of 69,664 Da based on their sequences. The intact hemoglobin comprises 12 subunits that fo...
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ژورنال
عنوان ژورنال: Comparative Biochemistry and Physiology Part A: Physiology
سال: 1997
ISSN: 0300-9629
DOI: 10.1016/s0300-9629(97)00224-7